increased plasma levels of soluble cd27 among hiv/hcv co-infected and hiv/hcv/gbv-c triply infected subjects

نویسندگان

alireza najafi department of immunology, faculty of medicine, shahid beheshti university of medical science, tehran

mostafa haji mollahoseini department of immunology, faculty of medicine, shahid beheshti university of medical sciences, tehran

shahram samiee research center laboratory, iranin blood transfusion organization, tehran

sedigheh amini kafi-abad research center laboratory, iranin blood transfusion organization, tehran

چکیده

cd27 is a biomarker associated with both t-cells and b-cells activation .plasma soluble cd27 (scd27) was identified as  a marker of disease outcome in human immunodeficiency virus (hiv) infection .testing of plasma scd27 represents a good tool to monitor the change of immune activation during hiv infection.we sought to analyses role of hepatitis c virus (hcv) and also gb virus type c (gbv-c) co-infections on hiv-related immune activation, through measuring scd27 plasma levels.blood samples from a total of 86 patients with hiv infection were taken. plasmas were analyzed for hcv using serologic test and gbv-c by reverse transcriptase polymerase chain reaction (rt-pcr). cd4+ and cd8+t-cell counts were evaluated by cd3/cd4+ and cd3/cd8+ double staining of whole blood followed by flow cytometric analysis .then  cross-sectional comparison of scd27 plasma levels was carried out among patients : hiv (n=20), hiv/ gbv-c (n=14), hiv/ (hcv) (n=26) and hiv/hcv/gbv-c (n=26).plasma level of scd27 was higher in hiv/hcv/gbv-c patients as compared to hiv mono-infected patients (p= 0.006) and based on results there was significant differences in the plasma levels of scd27 between hiv-infected individuals with and without hcv coinfection (p=0.017) and also correlation between scd27 and percent of cd4+t-cells was in highest level among hiv/hcv co-infected patients group [r= -0.59 (p=0.001)]. high levels of scd27 among hiv/hcv patients argues in favor of scd27 plasma level determination for monitoring of clinical features among hiv/hcv coinfected patients.

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عنوان ژورنال:
journal of paramedical sciences

جلد ۴، شماره ۲، صفحات ۰-۰

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